The movie also features many of the songs from the operetta which used lyrics by Hammerstein and Orbach and music by George Gershwin and Herbert Stothart.

Aniuta (Bernice Claire), known as The Flame, is a peasant girl who incites the people against the Czarist regime and the aristocracy through singing.

Prince Volodya (Alexander Gray) is the leader of a group of Cossack troops who falls in love with the girl, even though she is part of a revolution that is opposed to his social class.

Konstantin (Noah Beery) is a revolutionary who also falls in love with Aniuta, much to the anger of his lover, Natasha (Alice Gentle).

Song Of The Flame is notable as the film that was accompanied in its initial release by Warner Bros.' first ever Looney Tunes cartoon short, Sinkin' in the Bathtub.