It is found in throughout the Greater Antilles, in Cuba, Hispaniola (the Dominican Republic and Haiti), Jamaica, and Puerto Rico.

Color phases in this bat are indicators of age differences or bleaching due to high concentrations of ammonia in the roost.

[3] Sooty mustached bats are monestrous and uniparous most of the time with twinning rarely occurring.

The largest embryo reported weighed 1.8 grams (0.063 oz), or 30.2% of the female's body mass.

Raptors such as the American kestrel, red-tailed hawk and merlin are among a few of the sooty mustached bat's predators.