The Huntsville Depot located on the Norfolk Southern Railway line in downtown Huntsville is the oldest surviving railroad depot in Alabama and one of the oldest in the United States.

Completed in 1860, the depot served as eastern division headquarters for the Memphis and Charleston Railroad.

[1][2] Huntsville was occupied by Union forces in 1862 during the Civil War as a strategic point on the railroad and the depot was used as a prison for Confederate soldiers.

The Huntsville Depot saw its last regularly scheduled passenger train, Southern Railway's The Tennessean, on March 30, 1968.

In October 2024, museum officials said they were no longer involved with operations and the property had been returned to the control of the City of Huntsville, which was considering options for the building.