The Vipers wore an orange and black kit and primarily played their home matches at the Rose Bowl and the County Ground, Hove.

[3] Southern Vipers were partnered with Hampshire, Sussex, Berkshire, Buckinghamshire, Dorset, Oxfordshire and the Isle of Wight Cricket Board.

[4] The Vipers were originally formed in 2016 to compete in the Women's Cricket Super League, and won the inaugural competition, winning all but one of their group games, and then defeating Western Storm at the finals day held at Chelmsford by 7 wickets.

[5] In 2020, women's cricket in England was reformed, but the Southern Vipers brand was retained, and they won the first two editions of the 50-over Rachael Heyhoe Flint Trophy, beating Northern Diamonds in the final both times.

[28][29] At the end of the season, five Vipers players were given full-time domestic contracts, the first of their kind in England: Georgia Adams, Tara Norris, Paige Scholfield, Lauren Bell and Maia Bouchier.

[30] The following season, 2021, Southern Vipers competed in both the Rachael Heyhoe Flint Trophy and the newly-formed Twenty20 competition, the Charlotte Edwards Cup.

[32] In the Rachael Heyhoe Flint Trophy, Southern Vipers progressed directly to the final after topping the group, winning six of their seven matches.

[40] In the Charlotte Edwards Cup, they finished second in the group stage before defeating North West Thunder in the semi-final and The Blaze in the final.

[41] In the Rachael Heyhoe Flint Trophy, they topped the group stage with seven victories in fourteen matches, before defeating The Blaze in the final.