The southern grasshopper mouse is a robust, small, nocturnal species that typically forms monogamous pairs.

[4] In the arid regions inhabited by the southern grasshopper mouse, the Arizona bark scorpion is plentiful and avoided by most predators because of the very painful sting it can inflict.

[7] Specifically, the venom binds to a glutamic acid residue located within the channel protein,[7] thus preventing neuron action potentials from firing and acting as a type of temporary anesthetic and pain reliever for the mouse.

When this glutamic acid was replaced with hydrophilic glutamine residue, the venom did not bind with the protein, and the pain modulation ability was lost.

[4] Southern grasshopper mice are unique among other North American rodents in the family Cricetidae in that their diet consists mostly of arthropods.

[4] Moreover, males' unique pauses in their testicular activity during the breeding season might also contribute to the lower population densities in which they live.

[4] The natural habitat of southern grasshopper mice include hot, arid valleys, and scrub deserts.

[4] In a study done by Denise H. Frank and Edward J. Heske, the home range of southern grasshopper mice in the Chihuahuan Desert were observed.

[3] There are no currently known conservation issues associated with southern grasshopper mice, largely due to its wide distribution and tolerance for disturbances caused by arthropods.

[9] Live crickets were preferred to deceased specimens, which might suggest that prey movement might be a catalyst and cue for predatory attack.

[9] Highly aggressive behaviors were displayed towards laboratory mice in the same study, such as biting the back and tail of their prey.

[9] In their study, both McCarty and Southwick concluded that food deprivation and the type of prey affected the intensity and aggressiveness of the predatory attacks observed by southern grasshopper mice.