In addition, both termini of the Gatlinburg Bypass in Tennessee are at US 441, but that road does not carry a route number.

SR 331 terminates at the east end of the overlap of SRs 20, 24, and 26, which also serves as the terminus of Florida Truck Routes 24 and 26.

At this point, the truck routes turn west overlapping SR 222 and intersection Florida State Road 20.

cross over Fishing Creek on the Mayor Harry G. Bone Bridge and enters the Milledgeville city limits.

The highways travel north along South Wayne Street where it becomes the former northwestern terminus of SR 112 Spur.

After a railroad crossing and the intersection with Martin Luther King Junior Drive, the road curves northwest, serving as a commercial strip.

Further downtown, the route encounters the western terminus of SR 326 (Central Avenue).

Four blocks later, CR Old 441 turns from North Highland Street, west onto East Fifth Avenue.

A few blocks later, it runs along the south side of a large Methodist Church and then Donnelly Park between North Baker Street and the southern terminus of County Road 44B (North Donnelly Street), where Old 441 changes from East Fifth Avenue to West Fifth Avenue.

After the blinking light at North McDonald Street, it curves to the right and begins to run parallel to the same former Atlantic Coast Line Railroad line on the south side that it crossed over near Mount Dora Golf Club.

Though the road is on a hill here, it takes a slight downgrade along the tracks by the time it reaches the former intersections with West Sixth Avenue and Helen Street, but remains above track level until it reaches the intersection of Overlook Drive.

Here, it moves slightly below the tracks as it intersects Oakland Drive and Oakland Lane, which runs under a trestle with 8 feet (2.4 m) clearance, but quickly levels our as it approaches the intersection of County Road 452 (Lakeshore Drive).

West of Barrow Avenue, Old 441 makes a right turn from East Alfred Street to Lake Shore Boulevard, where it runs parallel to a rail trail that was originally part of the ACL line it ran parallel to in Mount Dora.

By 1960, the main highways were re-routed onto a direct line between Belleview and Lady Lake.

The former US 27 Alternate/US 441 Alternate begins on the west side of US 27/US 441 as Oak Road across the street from a gated community.

It immediately curves north, where it passed under a bridge that carries US 27/US 441, with no other access to its parent route besides Fennell Boulevard, which is across the street from another gated community.

It turns straight north as it enters Eastlake Weir, and continues to run along the lake curving west along the north side as it enters Ocklawaha, where it serves as both the southern terminus of County Road 464C (Southeast 135th Avenue), and later the southeastern terminus of County Road 464.

After passing near Lake Weir Seaplane Base, the road heads northwest again just before the intersection with Southeast 119th Court.

After this the road enters the Belleview city limits and intersects SR 35 (County Road 35), where it briefly becomes SR 25 and immediately crosses the CSX Wildwood Subdivision, part of the CSX S-Line.

North of there, the road remains generally at the same northwest angle passing by many of the farms and wooded areas typical of northwestern rural Florida.

Shortly after a used truck dealership across from the intersection of Northwest 89th Street is a dirt racing track previously known as the Ocala Speedway, now known as the Bubba Raceway Park[7] A gas station can be found south of Northwest 100th Street in Martin, but it is the most notable landmark along the route.

[8] Later it passes by the Marion County Correctional Institute, then passes through the grounds of the Lowell Correctional Institution[9] and across from the northern edge of that the Florida State Fire College[10] North of the territory of the Fire College the route ran over a bridge over a former Atlantic Coast Line Railroad line that in recent times has been truncated in the next town, which happens to be Lowell where US 441 Alternate curved straight north had an intersection with County Road 329.

US 441 Alternate continued this straight south-to-north pattern through Reddick where the road encounters an all-way stop intersection with County Road 316 which also provides access to I-75 from communities such as Citra, Fort McCoy, and Salt Springs.

The road passes the Millwood Cemetery, and then curves northwest once again before finally terminating at an at-grade interchange with US 441.