[2] Ambrosi's cave salamander is endemic to a small area of northwestern Italy, occurring at scattered locations in La Spezia province.

At other times it is active on the leaf litter near streams and on wet rocky outcrops in wooded valleys at altitudes of up to 1,250 metres (4,100 ft).

[2] Ambrosi's cave salamander is found in moist environments where it is active by night on the ground, sometimes scrambling around in low vegetation.

After an elaborate courtship routine, a clutch of a few eggs about 6 mm (0.2 in) long is laid in a concealed location where they are guarded by the female until they hatch directly into juvenile salamanders.

This is on the basis that it has a very restricted range, its total extent of occurrence being less than 5,000 square kilometres (1,900 sq mi), and suitable habitat may be declining locally.