The Spital Brook is a minor tributary of the River Lea in Hertfordshire, England.

The Spital Brook rises in Hoddesdonpark Wood and flows eastwards, passing through Barclay Park, the former Hoddesdon Common, on its journey to the Lea, while an unnamed parallel stream to the south has been diverted to form ornamental waterbodies located within the former parkland of Broxbournebury.

The stream crosses the Roman road, Ermine Street, before going under the A10 and Cock Lane.

Spital Brook's profile is relatively steep for a Hertfordshire water course, and loses approximately 50 metres of height over the 1 kilometre upper section.

Due to the brook's small catchment area, unless it is raining heavily, only the lower reach is likely to have water in it.