These snakes are primarily nocturnal, and are semi-arboreal with younger animals preferring to utilize elevated hiding spots more than adults.

[11][12] Found in most types of habitats, but prefers rocky hillsides and outcrops with crevices and caves.

[13] The Papua specimen was found behind discarded corrugated tin sheets beside a disused airstrip in Eucalypt savanna-woodland habitat dotted with numerous termite mounds.

Being the largest members of this genus, captive specimens will usually accept mice and other small rodents.

Spotted pythons are often kept as pets due to their small size, docile temperaments, strong feeding responses, resiliency and easy captive care.

The varying coloration between individuals also makes them quite attractive to many herpetoculturalists who work with and breed exotic reptiles.

Spotted pythons are also fairly easy to breed, though some individuals (mostly males) may refuse to eat for a few weeks when they are interested in mating.