Alexander Bartlet served as Session Clerk from then until his death in 1910, and Sunday school Superintendent until 1893.

St Andrew's was at that time part of the UPC's Presbytery of London, in Canada West.

The first building for St. Andrew's was erected in 1865 on the southeast corner of Victoria Avenue and Chatham Street.

Alexander Ferrier Kemp was called from St Gabriel's Church in Montreal as its second minister.

Gray, like his predecessor, left for the United States (Kalamazoo, Michigan), and later retired in Toronto.

James C. Tolmie (1893-1915) resigned from St Andrew's, having combined the pulpit when elected Member of Provincial Parliament for Windsor in 1914.

He went overseas with the Essex Fusiliers, and was the finalist in the provincial Liberal Party Leadership Convention in both 1919 and 1922.

Dr. Hugh M. Paulin arrived in late 1915 and served the congregation over thirty-five years until his death in October 1952.

The building also hosts a Chinese-speaking congregation, the Windsor Chinese Presbyterian Church, that holds weekly worship on Sunday afternoons.

On May 1, 2016, the congregation voted to end current operations, sell their building and property, and investigate other ministry opportunities in the downtown core due to financial concerns.