The church possesses some of the earliest figural stained-glass windows made in the United States, crafted by William Jay Bolton.

The organ was designed by Louis Robert [nl] and built by Ernest Skinner as his Opus #524, the gift to the church from parishioner and financier George Foster Peabody in memory of his brother.

[15] In 1979 the New York Landmarks Conservancy, a private, not-for-profit organization started a preservation program for the church called the St. Ann Center for Restoration and Arts.

Through the program's efforts the chancel was renovated, 64 stained glass windows restored and the exterior fence repaired.

[16] The church received a Sacred Sites Grant of $10,000 for brownstone façade and roof restoration of parish house wing in 2014.

The large parish hall wing and formerly fully leased community spaces suffer from active leaks, plaster failure and weather infiltration as a result of the ongoing deterioration of the exterior envelope.

[7] Since 2012, The Forum at St. Ann's Arts & Culture Series organizes fundraising events with exhibitions, film screenings, poetry and book readings, panel discussions and concerts for the ongoing restorations.