St. Anne's Church and Mission Site is a historic religious site on Down Street at the southern end of Indian Island in Old Town, Maine, United States.

[1] St. Anne's Church is located on the east side of Down Street, near the southern end of Indian Island, location of the Penobscot Indian Island Reservation.

It is a single-story wood frame structure, with a front-facing gable roof and clapboard siding.

The building has had a series of significant alterations, having been originally built with a small tower and belfry on its roof, with an entry in the western facade and a fanlight in the gable.

The tower's fourth stage has Palladian windows on each side, and its pyramidal roof is framed by pinnacles at the corners.