St. Luke's and St. Margaret's Church is a former parish of the Episcopal Diocese of Massachusetts in the Allston neighborhood of Boston.

The founders were associated with Church of the Advent in Boston, and St Margaret's was similarly Anglo-Catholic in practice and worship.

It was the only parish in the diocese founded under Bishop Phillips Brooks, the famous preacher and author of O Little Town of Bethlehem.

The combined parish continued to struggle, but it enjoyed a resurgence of attendance and activity in the 1980s and 1990s, beginning under the rectorship of Mary Glasspool (who would later become the first open lesbian bishop in the Anglican Communion).

In February 2010, the diocese announced that it was closing the mission, while retaining the property as a base for its "Micah Project", a residential internship program for young adults.

A window depicting the Last Supper is done in an English glass style by the studio of George W. Spence of Boston and is set in the chancel over the altar reredos.