St. Mark Seminary is a diocesan minor seminary operated by the Roman Catholic Diocese of Erie and located on a campus in the southeast corner of the city of Erie, Pennsylvania.

Seminarians in residence at the seminary pursue philosophy and liberal arts studies at Gannon University.

[1] Twenty-two acres (8.9 ha) were purchased for a new campus and buildings outside the southeast corner of the city.

[1] In 1972, Erie opened the seminary program at St. Mark's to seminarians from other dioceses, and accepted men studying for the priesthood in the dioceses of Greensburg, Pittsburgh, Altoona-Johnstown, Richmond, and Arlington.

Seminarians currently in formation at St. Mark's are studying for the dioceses of Erie, Altoona-Johnstown, Buffalo, Greensburg, and Rochester.