St. Ursula Academy, located in the East Walnut Hills neighborhood of Cincinnati, Ohio, is a Catholic college-preparatory high school for young women that offers an intensive four-year program in the fields of English, mathematics, science, social studies, French, Spanish, Latin, and religion.

The sisters had an enrollment of sixty-three pupils that first year when classes were held in a house they rented at Ingleside and McMillan.

The following year the school was in session at 1339 East McMillan in the Worcester residence, which the sisters had recently purchased.

St. Ursula Academy is now chartered as a four-year college preparatory high school, and grades seven and eight are located at the Villa.

With the generous support of alumnae, friends, and parents, the physical plant underwent an extensive renovation, enabling the school to accommodate an increasing number of students.

In April 2017, the nearby University of Cincinnati announced that its women's basketball and women's volleyball teams would play their home games in the 2017–18 school year at the St. Ursula Gymnasium & Convocation Center while the teams' normal home of Fifth Third Arena undergoes renovations.

[9] The one-on-one tablet PC program was introduced with the class of 2010 to aid in St. Ursula Academy's mission of preparing women for a changing world.