St. Bartholomew-by-the-Exchange was a church and parish in the City of London located on Bartholomew Lane, off Threadneedle Street.

Recorded since the 13th century, the church was destroyed in the Great Fire of London in 1666, then rebuilt by Sir Christopher Wren.

St Bartholomew-by-the-Exchange was dedicated to the apostle who, by tradition, was martyred in Armenia by being flayed alive.

In the interim between this date and the building of the Royal Exchange, the church later called “St Bartholomew-by-the-Exchange” became lytyll saynt Bathellmuw besyd sunt Antony's.

In 1509, a south chapel was added by Lord Mayor and Draper Sir William Capel, whose mansion was in the parish.

In order to improve access to the site of the Exchange, the Corporation of London petitioned Parliament for permission to demolish St. Bartholomew-by-the-Exchange – as well as the neighbouring St Benet Fink, so that Threadneedle Street could be widened.

The removal of the numerous bodies buried in the church and churchyard began the week after, under he supervision of Mr Toplis, of New Bridge Street.

Upwards of a thousand bodies were removed with great care and decorum, including that of ecclesiastical reformer Myles Coverdale buried in 1569.