The state highway runs 8.39 miles (13.50 km) from Interstate 81 (I-81) in Chilhowie north to SR 91 in Saltville in western Smyth County.

SR 107 begins at the south end of its diamond interchange with I-81 on the southern edge of the town of Chilhowie.

The state highway heads north as two-lane undivided White Top Avenue.

SR 107 crosses over the Middle Fork Holston River, has a grade crossing of Norfolk Southern Railway's Pulaski District, and intersects U.S. Route 11 (Lee Highway) one block north of Chilhowie's Main Street.

On entering the town of Saltville, the state highway follows Worthy Boulevard, which passes through a sweeping S-curve.