It is biosynthesized from alpha-linolenic acid (ALA: C18H30O2; 18:3, n-3) by the enzyme delta-6-desaturase, which removes two hydrogen (H) atoms.

[1] As it is a precursor to other fatty acids, there has been efforts to enhance the content off stearidonic acid in various crops, such as soybeans.

[2] SDA is also a precursor to N-acylethanolamine (NAEs).

[3][4] Natural sources of this fatty acid are the seed oils of hemp, blackcurrant, corn gromwell,[5] and Echium plantagineum, and the cyanobacterium Spirulina.

A GMO soybean source is approved by the European Food Safety Authority.