Goal-line dropouts and the ability to replace a red carded player after 20 minutes were carried over to this competition as were used in both Super Rugby AU and the 2021 Super Rugby Aotearoa season.

The captain's referral and Golden Point extra time used in the 2021 Super Rugby Aotearoa season though were not used in Super Rugby Trans-Tasman, while the 50/22 kicking law, restart variation and Golden Try extra time used in the 2021 Super Rugby AU season were also not used in the competition.

[5] The table below shows the progression of all teams throughout Super Rugby Trans-Tasman.

Players listed in italics denote non-original squad members.

The following referees were selected to officiate Super Rugby Trans-Tasman:[36][37][38][39][40][41][42] In Australia, all games were broadcast live on streaming service Stan Sport, with a select game each round simulcast live free-to-air on the Nine Network.