The women's 100 metre freestyle competition at the 2007 World Aquatics Championships was held on 29 and 30 March 2007 at the Rod Laver Arena in Melbourne, Australia.
135 swimmers were entered in the event.
Prior to the competition, the existing world and championship records were as follows.
[1] The following records were established during the competition: * Split from the women's 4 × 100 m freestyle relay