The women's 100 metre breaststroke event in swimming at the 2013 World Aquatics Championships took place on 29–30 July at the Palau Sant Jordi in Barcelona, Spain.

[1][2] Prior to this competition, the existing world and championship records were: The following new records were set during this competition.

The heats were held at 10:30.

[6] The semifinals were held at 18:29.

[7] The final was held at 19:48.