The women's 50 metre breaststroke event in swimming at the 2013 World Aquatics Championships took place on 3–4 August at the Palau Sant Jordi in Barcelona, Spain.

[1][2] Prior to this competition, the existing world and championship records were: The following new records were set during this competition.

† – en route to final mark The heats were held at 10:32.

[6] The semifinals were held at 18:26.

[7] The final was held at 18:08.