The women's 400 metre freestyle S9 event at the 2016 Paralympic Games took place on 9 September 2016, at the Olympic Aquatics Stadium.

Three heats were held.

The swimmers with the eight fastest times advanced to the final.

10:57 9 September 2016:[1] 11:04 9 September 2016:[1] 11:12 9 September 2016:[1] 19:51 9 September 2016:[2]