The U.S.' teen Lydia Jacoby upset South Africa's Tatjana Schoenmaker and defending champion Lilly King to capture the sprint breaststroke title.
First at the turn, Schoenmaker could not contend with Jacoby's blistering final lap and settled for silver in a time of 1:05.22, 0.4 seconds shy off her Olympic record in the heats.
ROC's Evgeniia Chikunova delivered a time of 1:05.90 to pick up the fourth spot, just ahead of teammate and defending silver medallist Yuliya Yefimova (1:06.02) by about a tenth of a second.
Sweden's Sophie Hansson placed sixth in 1:06.07, while Martina Carraro (1:06.19) and Ireland's Mona McSharry (1:06.94) rounded out the championship field.
Up to one swimmer per NOC meeting that time is eligible for selection, allocated by world ranking until the maximum quota for all swimming events is reached.