The women's 100-metre breaststroke competition at the 2023 World Aquatics Championships was held on 24 and 25 July 2023.

[1][2][3] Prior to the competition, the existing world and championship records were as follows.

[4] The heats were started on 24 July at 11:09.

[5] The semi-finals were started on 24 July at 20:28.

[6] The final was held on 25 July at 21:45.