The Women's 100 metre breaststroke competition at the 2024 World Aquatics Championships was held on 12 and 13 February 2024.

[1][2] Prior to the competition, the existing world and championship records were as follows.

[3] The heats were started on 12 February at 10:04.

[4] The semifinals were held on 12 February at 19:28.

[5] The final was held on 13 February at 20:45.