Each series is represented by characters, backgrounds, music, and voice actors from the original television programs, along with new art and animated sequences produced by Tatsunoko Production.

[1] In the future, a young scientist named Battering creates the "Salvasion System", a dimensional mobile device which allows for travel between parallel worlds.

To stop this, Battering created a combat strengthening suit to become Volter the Lightning and threw himself into the parallel worlds to unite with the Tatsunoko heroes and defeat Dokucyber and the Jaleizer Empire.

[2] The game relies on using both normal and special attacks in order to damage the opponent, with a round ending once all of a character's remaining vitality has been lost.

[3] Tatsunoko Fight features playable characters from four established Tatsunoko franchises: Science Ninja Team Gatchaman, Neo-Human Casshern, Tekkaman: The Space Knight, and Hurricane Polymar, as well an original series created exclusively for the game, Denkou Senka Volter (電光石火ヴォルター, literally "Lightning Warrior Volter").