The United States' Bethanie Mattek-Sands and Jack Sock defeated compatriots Venus Williams and Rajeev Ram in the final, 6–7(3–7), 6–1, [10–7] to win the gold medal in Mixed Doubles tennis at the 2016 Summer Olympics.

In the bronze-medal match, the Czech Republic's Lucie Hradecká and Radek Štěpánek defeated India's Sania Mirza and Rohan Bopanna, 6–1, 7–5.

The tournament was held at the Olympic Tennis Centre in the Barra Olympic Park in Barra da Tijuca in the west zone of Rio de Janeiro, Brazil from 10 to 14 August 2016.

Tennis was not a medal sport from 1928 to 1984, though there were demonstration events in 1968 (which included mixed doubles) and 1984 (which did not).

Teams had to be nominated by their National Olympic Committee by the deadline of 9 August.