The main belt consists of oaklands and savannas and runs from just south of the Red River on the Texas-Oklahoma border through the Dallas–Fort Worth metropolitan area and southward to the vicinity of San Antonio, Bexar County.

Native American hunter-gatherers contributed to the maintenance of the prairie through controlled burns to make more land suitable for hunting bison and other game.

By the end of the 19th century 98% of the blackland prairies were cultivated and it was the leading cotton region of the state, also producing sorghum, corn, wheat, and hay.

Several of the larger cities in the state, such as Austin, Dallas, Fort Worth, San Antonio, Temple, and Waco, are located in the region and the agricultural activity in the area was a significant factor in their growth in the 19th century.

Herds of bison, and to a lesser extent pronghorn and deer, grazed on the grasses and trampled and fertilized the soil, stimulating the growth of the tallgrass ecosystem.

[10] The rich black 'waxland' soil of these prairies is almost proof against burrowing rodents, which penetrate the region only along some sandy stream bottoms, while the open country tempts jack rabbits, coyotes, and other plains species eastward slightly beyond their usual bounds.

[7]: 19 p. The negative effect on distribution is evident in the Texas distribution of many wide-ranging species of both eastern and western North American fauna reaching their respective distributional limits in the region of the Texas Blackland Prairies and East Central Texas forests [e.g. eastern: American alligator (Alligator mississippiensis), eastern gray squirrel (Sciurus carolinensis), southern flying squirrel (Glaucomys volans),; western: American badger (Taxidea taxus), western diamondback rattlesnake (Crotalus atrox); and other fauna listed below].

Mammals: Some species found in the region include Virginia opossum (Didelphis virginiana), nine-banded armadillo (Dasypus novemcinctus), Eastern red bat (Lasiurus borealis), Brazilian free-tailed bat (Tadarida brasiliensis), fulvous harvest mouse (Reithrodontomys fulvescens), white-footed mouse (Peromyscus leucopus), hispid cotton rat (Sigmodon hispidus)), eastern fox squirrel (Sciurus niger), American beaver (Castor canadensis), white-tailed deer (Odocoileus virginianus), northern raccoon (Procyon lotor), striped skunk (Mephitis mephitis), bobcat (Lynx rufus), gray fox (Urocyon cinereoargenteus), and coyotes (Canis latrans).

[11][12] The large, keystone species that once inhabited the Blackland Prairies, before the arrival of Europeans and the destruction of the tallgrass ecosystem, are now extirpated, including American bison (Bos bison), gray wolf (Canis lupus), red wolf (Canis rufus), mountain lion (Puma concolor), black bear (Ursus americanus), and pronghorn (Antilocapra americana) which once ranged into the western areas, and even jaguar (Panthera onca) and ocelot (Leopardus pardalis) that once occurred in the southern regions.

Various seasonal and migratory species appearing in the region include Bufflehead (Bucephala albeola), Hooded Merganser (Lophodytes cucullatus), Little Blue Heron (Egretta caerulea), Yellow-crowned Night Heron (Nyctanassa violacea), Northern Harrier (Circus hudsonius), Inca Dove (Columbina inca), Yellow-billed Cuckoo (Coccyzus americanus), Ruby-throated Hummingbird (Archilochus colubris), Scissor-tailed Flycatcher (Tyrannus forficatus), American Pipit (Anthus rubescens), Orange-crowned Warbler (Leiothlypis celata), Blue Grosbeak (Passerina caerulea), Indigo Bunting (Passerina cyanea), Painted Bunting (Passerina ciris), Harris's Sparrow (Zonotrichia querula), and Dickcissel (Spiza americana).

[13][14] A few of the year round resident species include wood duck (Aix sponsa), blue-winged teal (Anas discors), black vulture (Coragyps atratus), cooper's hawk (Accipiter cooperii), red-shouldered hawk (Buteo lineatus), American kestrel (Falco sparverius), great horned owl (Bubo virginianus), barred owl (Strix varia), greater roadrunner (Geococcyx californianus), belted kingfisher (Megaceryle alcyon), red-bellied woodpecker (Melanerpes carolinus), hairy woodpecker (Leuconotopicus villosus), loggerhead shrike (Lanius ludovicianus), red-winged blackbird (Agelaius phoeniceus), eastern meadowlark (Sturnella magna), eastern bluebird (Sialia sialis), tufted titmouse (Baeolophus bicolor), Carolina wren (Thryothorus ludovicianus), chipping sparrow (Spizella passerina), lark sparrow (Chondestes grammacus), and grasshopper sparrow (Ammodramus savannarum).

[15][16] Snakes are the most diverse group of reptiles in the region, species include the North American racer (Coluber constrictor), eastern hog-nosed snake (Heterodon platirhinos), prairie kingsnake (Lampropeltis calligaster), speckled kingsnake (Lampropeltis holbrooki), coachwhip (Masticophis flagellum), yellow-bellied watersnake (Nerodia erythrogaster), diamond-backed watersnake (Nerodia rhombifer), rough greensnake (Opheodrys aestivus), western ratsnake (Pantherophis obsoletus), Graham's crayfishsnake (Regina grahamii), flat-headed snake (Tantilla gracilis), western ribbonsnake (Thamnophis proximus), and several others.

[17][18][19] Just a few of the smaller species include threadfin shad (Dorosoma petenense), red shiner (Cyprinella lutrensis), blacktail shiner (Cyprinella venusta), shoal chub (Macrhybopsis hyostoma), ghost shiner (Notropis buchanani), pugnose minnow (Opsopoeodus emiliae), fathead minnow (Pimephales promelas), bullhead minnow (Pimephales vigilax), river carpsucker (Carpiodes carpio), blackstripe topminnow (Fundulus notatus), slough darter (Etheostoma gracile), bigscale logperch (Percina macrolepida), and dusky darter (Percina sciera) and many others.