The Girlhood of Mary Virgin is an 1849 oil on canvas painting by the Pre-Raphaelite Brotherhood painter Dante Gabriel Rossetti, measuring 83.2 by 65.4 cm and now in the collection of Tate Britain, to which it was bequeathed in 1937 by Agnes Jekyll.

The angel's face was originally based on the half-sister of Thomas Woolner (1825-1892), but in August 1849 Rossetti replaced her features with those of another girl, recommended to him by his fellow Pre-Raphaelite James Collinson (1825-1881).

Rossetti wrote two sonnets to explain the painting's symbolism — the first was engraved at the bottom of the original frame, whilst the second was printed in the Free Exhibition catalogue.

Rossetti repainted Mary's dress and the angel's face before sending the work to her, whilst the payment for it funded a trip to Belgium and France by him and William Holman Hunt.

By 1864 it was owned by Lady Louise Fielding, who that year sent it back to Rossetti for repainting — he turned the angel's wings from white to deep pink and Mary's sleeves from yellow to brown.