The 2,750-hectare (6,800-acre) reserve, which was gazetted in January 1999, is situated approximately 25 kilometres (16 mi) south-east of Gloucester, off Bucketts Way.

The reserve is predominantly sclerophyll forest, and contains many endangered species of flora and fauna.

The major vegetation types are Eucalyptus and Angophora, with many species of fern and orchid.

The fauna of The Glen Nature Reserve is well studied, and includes a large biodiversity.

There are many endangered species of animal within the nature reserve, including the koala (Phascolarctos cinereus) and the yellow-bellied glider (Petaurus australis).