The charity has eight regional centres in Belfast, Cardiff, Forfar, Leeds, Atherton, Leamington Spa, Redbridge and Bristol.

There are a further 14 community teams in Edinburgh, Glasgow, Newcastle, Hull, Liverpool, Nottingham, Shrewsbury, Birmingham, Welwyn, central London, Maidstone, Reading (based at head office), Southampton and Exeter.

At six weeks old, the puppies undergo health checks and vaccinations at Guide Dogs' National Centre in Leamington Spa, Warwickshire.

[8] Buddy dogs help children and young people who are blind or partially sighted to build confidence in themselves and trust in their surroundings.

Guide Dogs provides the following services to help children and young people with sight loss: My Time to Play – launched in 2020, this service comprises online and face-to-face sessions to help children aged 0–4 to develop through play, and their parents can also connect with other families affected by sight loss.

Tech for All – In 2021, Guide Dogs is piloting a scheme giving those aged three to 18 with a vision impairment a free iPhone or iPad.

The project was launched after the charity's own research found that technology is a vital tool for people who are blind or partially sighted.

The charity has a research programme to provide an evidence base for Guide Dogs' policies, operational procedures and campaigns.

The research is carried out internally and in collaboration with external organisations, helping the charity to understand its service users' needs and care for its dogs.

A few years later, a breeding programme was introduced and by 1970, these components of Guide Dogs' work had grown so much they were given their own premises at Tollgate House, near Leamington Spa.

[11] In 1964, the children's television programme Blue Peter followed the training of two guide dog puppies, Cindy and Honey.

In 2021, the charity celebrated the 90th anniversary of the UK's first four guide dog partnerships with a host of activities including an artisan sensory garden at the RHS Chelsea Flower Show.