The Trail of the Lonesome Pine is a 1916 American silent drama film directed by Cecil B. DeMille, who also wrote the screenplay.

Charlotte Walker reprised her role from the Broadway production.

Another version released in 1923 starred Mary Miles Minter and is now considered a lost film.

The novel was adapted for the fourth time in 1936, an early Technicolor version starring Fred MacMurray, Sylvia Sidney, and Henry Fonda.

Complete 35 mm prints of The Trail of the Lonesome Pine are held by the Library of Congress and the George Eastman Museum in Rochester, New York.