Bot was called to active duty and served in an air defense artillery platoon during the Dutch East Indies campaign.

Bot worked as political advisor for the High Commissioner of the Dutch East Indies Tony Lovink from March 1949 until December 1949.

Bot served as Deputy Secretary-General of the Netherlands-Indonesian Union and as a political consultant for the Ministry of Colonial Affairs from December 1949 tot March 1954.

Bot remained in active in national politics, in September 1967 he was nominated as Ambassador to Canada, taking office on 17 January 1968.

Bot retired after spending 16 years in national politics and became active in the public sector and occupied numerous seats as a nonprofit director on several boards of directors and supervisory boards (UNICEF, United Nations Commission on Science and Technology for Development (CSTD), Oxfam Novib and the Transnational Institute) and served on several state commissions and councils on behalf of the government (Kadaster, Public Pension Funds PFZW, Sociale Verzekeringsbank and KPN) and served as a diplomat and lobbyist for several economic delegations on behalf of the government.

Bot continued to comment on political affairs until his is death at the age of 73 and holds the distinction as the first serving Minister for Development Cooperation.

[1] Bot served as State Secretary of the Interior, in charge of matters concerning Netherlands New Guinea, from 23 November 1959 until 24 July 1963 in the De Quay cabinet.

After his membership of the cabinet he was appointed Dutch ambassador in Ottawa, Ontario, Canada in September 1967, serving from 17 January 1968 until July 1973.