The railway from Thessaloniki to Bitola is a 219 kilometres (136 mi) long railway line, that connects the port city Thessaloniki in Greece with Bitola in the Republic of North Macedonia, via Veroia, Edessa, Amyntaio and Florina.

The easternmost section of the line, Platy–Thessaloniki, is part of the important connection towards Athens and Northern Greece.

Leaving the Athens–Thessaloniki mainline at Platy, it runs alongside the Aliakmon River, through Alexandreia and then passes through to Veroia, Naousa, and Skydra, before climbing to Edessa and then, along the northern shore of Lake Vegoritida, reaching Amyntaio.

At Neos Kafkasos, the international border is crossed, and after 219 km the city of Bitola in the Republic of North Macedonia is reached.

While at that time, the practice of compulsory work was still in use, labour was cheap in rural Macedonia, and the engineers were reported to be enthusiastically welcomed.