[5] Eurycea guttolineata is a mid-sized, slender stream salamander which ranges from about 10-15.9 cm in its adult form.

It is tan to light yellow with three black longitudinal stripes running from the eyes down the length of the body to the tail.

Additionally, the ventrum (belly) of the three-lined salamander is boldly marked with black and white marbling.

[7] Due to this species being a semi-aquatic salamander in the Plethodontidae family, the females will enter cooler waters in late autumn and winter to drop eggs.

[11] It can be found in the Appalachian Mountains from Virginia and Tennessee south through the Carolinas, Georgia, Alabama, and Mississippi to the Gulf Coast, including eastern Louisiana and western Florida.

[12] Larvae are thought to feed on small invertebrates, but there have not been any detailed studies on their stomach content or foraging behavior.