In addition it is very important for rare species of tugay, or riparian forest, ecosystems.

[2] The climate is continental and dry, the different habitats of Tigrovaya Balka comprise semideserts, savanna-like grasslands with pistachio trees and tugay vegetation with poplars, Russian silverberry and high grasses.

[3] As of today, Tigrovaya Balka is still home to the original main prey of the tiger, the rare Bactrian deer.

Other larger species of the reserve include striped hyenas, golden jackals, swamp cats, wild boars, goitered gazelles, porcupines, the introduced nutrias,[3] gray wolves, red foxes and in hills also urials.

These include red-crested pochards, pygmy cormorants, saker falcons, common coots, common cranes, pale-backed pigeons, pallid scops-owls, Egyptian nightjars, white-winged woodpeckers, brown-necked ravens, great tits, desert larks, streaked scrub-warblers, Sykes's warblers, Asian desert warblers, saxaul sparrows and desert finches.