[1][2] This very small sinkhole lake contains quite clear water that generally ranges between 18 and 25 °C (64 and 77 °F) depending on season,[3] and the Otjikoto tilapia has been seen down to depths of 67 m (220 ft).

[3] The Otjikoto tilapia mostly feeds on algae, including diatoms,[2] but it is an opportunistic omnivore and will also take invertebrates.

[5] There are minor genetic differences between the morphs and assortative mating occurs, especially in the olive and dark blue (possibly showing the very early stages of separation into distinct species).

[5] Some pale individuals have a highly mottled appearance, giving the species the nicknames African koi and Nguni fish.

[4] It is very closely related to the banded tilapia (T. sparrmanii) and the two species can interbreed.