The Tilburg–Nijmegen railway is a railway line in the Netherlands running from Tilburg to Nijmegen, passing through 's-Hertogenbosch.

The line was opened in 1881.

The main interchange stations on the Tilburg–Nijmegen railway are: It has been proposed that the Berkel-Enschot railway station should be reconstructed, adding a station between 's-Hertogenbosch and Tilburg.

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