Tlustice is a municipality and village in Beroun District in the Central Bohemian Region of the Czech Republic.

The highest point is the hill Tlustický vršek at 402 m (1,319 ft) above sea level.

The first written mention of Tlustice is from 1320, when King John of Bohemia donated the village to Zbyněk Zajíc (later known as Zbyněk Zajíc of Hazmburk), who annexed the village to his Žebrák estate.

In 1611, Jan Litvín Kavka of Říčany bought Tlustice and annexed it to the Hořovice estate.

As a result of the Bohemian Revolt, Jan Kavka's properties were confiscated in 1622 and the Hořovice estate was acquired by the Martinic family.