[1] At the time of its operation it was one of three museums dedicated to cartoon art in the United States.

[2] The ToonSeum moved to its own gallery space on Liberty Avenue in Pittsburgh's downtown Cultural District on November 8, 2009, aided by the Pittsburgh Cultural Trust.

[3] In 2009, the ToonSeum established its NEMO Award, given to notable individuals "for excellence in the cartoon arts".

[6] In May 2013, the ToonSeum hosted the two-day North American Conference of the National Cartoonists Society.

[8] On February 16, 2018, the leadership of the ToonSeum announced via the organization's Facebook page that it would close the physical location on Liberty avenue February 24, 2018 to focus on direct community outreach.