In 1985, the club was founded as Broxbourne Ladies by Sue Sharples and Kay Lovelock, following the folding of East Herts College.

[9] At the start of the 2017–18 campaign, the club made high-profile signings such as former England U20 midfielder Coral-Jade Haines and Wales international forward Sarah Wiltshire.

The season saw the team claim their first ever victory over a top flight side, beating Bristol City in their FA Women's League Cup matchup.

In March 2018, it was confirmed that the club would apply to remain in the newly re-branded Women's Championship and would compete in the upcoming season.

[14] After a 1–1 draw with Aston Villa on 1 May 2019, Spurs secured a promotion spot to the FA Women's Super League for the first time in their history.

[16] On 17 November 2019, the North London derby against Arsenal at the Tottenham Hotspur Stadium recorded an attendance figure of 38,262, the highest ever for a Women's Super League match at the time.

[20][21] Still trying to regain fitness having not played since August 2019 and giving birth in May 2020,[22] Morgan eventually made her Spurs debut nearly two months after signing, on 7 November, appearing as a 69th minute substitute in a 1–1 WSL draw against Reading.

Due to this form, Rehanne Skinner was appointed as the new head coach of Tottenham on 19 November 2020, replacing long time managers Karen Hills and Juan Amorós.

[24][25] Hills had been in charge of the first team since August 2009 while Spurs were playing in the then-third tier South East Combination Women's Football League and Amoros joined the club soon after in February 2011.

Spurs would beat Liverpool in the quarter-finals to advance to their first ever semi-final in any major cup competition, but would bow out after a loss to eventual champions Manchester City.

Their final match at White Hart Lane on 19 April 2017 saw them win the FA Women's Premier League South title against rivals West Ham United Ladies.

Tottenham Hotspur Women also run a football academy in partnership with Barnet and Southgate College for girls aged 16–19.