It was in existence from 1927 to 1959 and its member schools were affiliated with the Wisconsin Interscholastic Athletic Association.
The Trempealeau Valley Conference was formed in 1927 by six small high schools in close proximity to the Trempealeau River in the Driftless Area of Wisconsin: Alma Center, Blair, Hixton, Independence, Taylor and Whitehall.
[4] In 1949, Alma Center and Merrillan merged with Humbird to form the new Lincoln High School in Alma Center.
[5] Eleva-Strum (also known as Central Union)[6] joined the Trempealeau Valley in 1952,[7] giving the conference seven members for the rest of its history.
Four schools entered the West Central Conference (Alma Center Lincoln, Fairchild, Hixton and Taylor)[8] and three became members of the Dairyland Conference (Blair, Eleva-Strum and Independence).