The Tryphoninae comprise a worldwide subfamily of the parasitic wasp family Ichneumonidae.

Most species of the Tryphoninae are koinobiont ectoparasitoids of Symphyta larvae, but members of some genera (e.g. Netelia) are ectoparasitoids of Lepidoptera larvae.

Tryphonines have a hair-margined clypeus and two longitudinal parallel ridges occur on the first tergite.

The female sometimes has stalked eggs projecting from her ovipositor.

As of 2017[update], the following seven tribes are recognized.