In 1216 the Danish king Valdemar II handed over control of Tunø to the bishop of Aarhus.

Inside Tunø church the minister and parish clerk's seats, which date from around 1520, are carved with the coat of arms of Niels Clausen Skade, the then bishop of Aarhus.

The sepulchral tablet on the north wall of the choir has a fresco depicting the vicar Jørgen Hansen, who was said to have been tossed by a bull in 1640.

The triptych depicts the Virgin Mary as heavenly Queen perched on a crescent holding the Baby Jesus in her left arm whilst holding a bunch of grapes in her right hand.

It is said that prior to the foundation of the church, Tunø's residents would sail to nearby Samsø to attend Nordby Kirke, whose bricked up northern door is referred to as Tunboernes dør or Tunø islander's door.