The site occupies part of a disused quarry, in which have developed areas of primary and secondary magnesian limestone grassland.

The grasslands at Tuthill Quarry are typical of the type, being characterised by the presence of blue moor-grass, Sesleria albicans, and small scabious, Scabiosa columbaria, but a number of less common species are also present, including common butterwort, Pinguicula vulgaris, and adder's-tongue fern, Ophioglossum vulgatum.

There is a small patch of the nationally scarce bird's-eye primrose, Primula farinosa, and one of the few records from lowland Durham of lesser clubmoss, Selaginella selaginoides, is from this site.

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