The stadium has hosted concerts by artists such as Rihanna, Iron Maiden, Bryan Adams, Bon Jovi, Genesis, U2, Beyoncé, the Rolling Stones, the Police, Eagles, R.E.M., Eminem, Lady Gaga, Metallica, Depeche Mode and NSPPD Prayer Conference.

[5] The stadium, owned and operated by the RFU, hosts rugby union fixtures all year round.

Twickenham hosts England's home Six Nations matches, as well as games against touring teams from the Southern Hemisphere, usually annually in November.

Apart from its relationship with the national team, Twickenham is the venue for a number of other domestic and international rugby union matches.

After further expenditure on roads, the first game, between Harlequins v. Richmond, was played on 2 October 1909, and the first international, England v. Wales, on 15 January 1910.

[8] In 1959, to mark 50 years of the ground, a combined side of England and Wales beat Ireland and Scotland by 26 points to 17.

This day also saw the origins of the adoption of the traditional spiritual "Swing Low, Sweet Chariot" as a terrace song.

Three of England's tries in the match were scored by Chris Oti, a black player who had made a reputation for himself that season as speedy left winger.

During this match, with the English facing a 12–3 deficit, David Campese reached one-handed for a ball thrown to England winger, Rory Underwood.

Under the reign of Clive Woodward, the stadium became known as 'Fortress Twickenham', as England enjoyed a run of 19 unbeaten home matches from October 1999, ending with defeat against Ireland in 2004.

The IRB Rugby Aid Match was played on 5 March 2005 under the auspices of the International Rugby Board (IRB) to raise money for the United Nations World Food Programme to support its work helping victims of the 2004 Indian Ocean tsunami.

In 2008, Harlequins once again became tenants at Twickenham for selected matches including the annual Big Game fixture usually hosted in late December each year.

[13] In May 2023 during the 2023 Premiership Rugby Final between Sale Sharks and Saracens a group of Just Stop Oil protesters came onto the pitch and started to vandalise the surface.

It was found to be cheaper to build a new stand than to repair the existing one; however, planning permission was refused, due to objections from local residents.

[16] Planning permission was sought in 2002 and received in December 2004 for a new South Stand to further raise capacity, with building work commencing in June 2005.

As well as increasing the stadium's capacity to 82,000, the redevelopment introduced a four-star Marriott hotel with 156 rooms and six VIP suites with views over the field, a performing arts complex, a health and leisure club, a new rugby shop and a general increase in function space.

[17] The east stand was over budget due to additional safety measures put in place to make the redeveloped structure capable of withstanding a bomb attack, and to make it fireproof, with work carried out on the cladding in response to the Grenfell Tower fire.

[21] The Stones also played two shows at Twickenham in August and September 2003, the first of which was used as their stadium concert disc for the 2003 DVD Four Flicks.

performed at Twickenham in August 2008, while New Jersey rockers Bon Jovi played two gigs at the stadium in June 2008 as part of their Lost Highway Tour, and Iron Maiden played there as part of their Somewhere Back in Time World Tour on 5 July 2008, along with a full supporting bill which included Avenged Sevenfold, Within Temptation and Lauren Harris.

It was announced on 3 November 2015 that the RFU and America's National Football League had agreed a three-year deal to host at least three NFL London Games.

It tells the history of the sport, including William Webb Ellis and Richard Lindon, using interactive display techniques.

The museum has a rolling programme of special exhibitions which cover topical issues and offer an opportunity to display some of the obscurer items in the collection.

Twickenham Stadium Tours are also available through the Museum and run four times per day (Tuesday to Saturday) and twice on Sundays.