Operating New London, Connecticut, in 1923, S-25 participated in winter maneuvers in the Caribbean Sea and the Panama Canal Zone area from January to April 1924.

Then transferred to the West Coast of the United States, she operated primarily in the waters off Southern California until 1931.

S-25 cleared Pearl Harbor to return to the United States East Coast on 16 June 1939 and arrived at New London on 25 August 1939.

The Polish Navy commissioned her immediately as ORP Jastrząb for World War II service.

While traveling in an Allied convoy near Norway, Jastrzab strayed some 100 nautical miles (190 km; 120 mi) off her proper course[citation needed], and on 2 May 1942 was mistakenly sunk by friendly fire by the British destroyer HMS St Albans (ex-USS Thomas (DD-182)) and minesweeper HMS Seagull.