It was situated in the Polish–Lithuanian Commonwealth between the rivers Bug and Styr on territory that is today located in parts of the modern states of Ukraine and Belarus.
The eparchy was a suffragan of the Metropolis of Kiev, Galicia and all Ruthenia.
Following the partitions of Poland, the territory of the eparchy was absorbed by the Tsardom of Russia.
Consequently, in 1795 the eparchy was suppressed and the incumbent ordinary — Simeon Młocki — was obliged to resign.
It was partially reestablished in 1798 when the Eparchy of Brest was erected on 12 October 1798; Josaphat Bulhak was appointed as the first eparch.