It is located at the junction of Avenida Libertador General Bernardo O'Higgins, also known as "Alameda", and Paseo Ahumada in the commune of Santiago.

Other landmarks near the station include the Paseo Ahumada, a four-block pedestrian shopping street; the Instituto Nacional General José Miguel Carrera, or simply "Instituto Nacional", Chile's oldest and most prestigious school; Barrio París-Londres, San Francisco Church, the Santiago Stock Exchange, La Moneda Palace and the main headquarters of some of Chile's oldest banks.

[2] Universidad de Chile Station features a 1200 square meter mural called "Memoria Visual de una Nación" (Visual Memory of a Nation), painted by Mario Toral, a Chilean painter and photographer.

It shows fragments of Chile's history, from the violent Spanish conquest to the modern day.

In 2011, Lonely Planet named Universidad de Chile one of its "Subway stations worth getting off the train for",[3] and in 2014, the BBC named it one of the seven most beautiful stations in the world.